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Effects of PH in the reaction medium, the incubation temperature, the extracting agent and the sample matrix on the detection sensitivities were also studied and discussed.

Preparation of acellular nerve matrix using Trito X-100 and sodium deoxycholate as extracting agent: Is there an optimal time?

Method: Respectively degree of orange peel thickness and weight, extracting agent and extraction time for mixing crushed were performed single factor experiment, respectively.

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